DNA

Part:BBa_K2796029:Design

Designed by: Shuangshuang Pu   Group: iGEM18_LZU-CHINA   (2018-10-04)


hsa-mir-135b-3p

    The expressed plasmid map of this part is shown below:
    2018 LZU-CHINA 29design2.png

    Agarose gel electrophoresis

    We used pCDH plasmid to overexpress miR135b3p in gastric cancer cells and used the mRFP reporting system to detect the transfection efficiency. The result of agarose gel electrophoresis for miR-135b-3p, miR-942b-5p and miR-769-5p is shown below.

    2018 LZU-CHINA 29experimental Gel.png



    Assembly Compatibility:
    • 10
      COMPATIBLE WITH RFC[10]
    • 12
      COMPATIBLE WITH RFC[12]
    • 21
      COMPATIBLE WITH RFC[21]
    • 23
      COMPATIBLE WITH RFC[23]
    • 25
      COMPATIBLE WITH RFC[25]
    • 1000
      INCOMPATIBLE WITH RFC[1000]
      Illegal BsaI.rc site found at 34


    Design Notes

    no


    Source

    NCBI

    ===References===